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Recombinant Human Fibroblast growth factor 2 (FGF2) Protein


生產種屬

Human (Homo sapiens)

來源

Prokaryotic expression

宿主

E.coli

同義詞

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

濃度

1 mg/mL

形態

Liquid

純度

≥85% by SDS-PAGE

序列

143-288aa (P09038)

標簽

EP, N-terminal 6xHis-tagged

純化方式

Affinity purification

分子量

20.4 KD

應用

Western blotting,ELISA;SDS-PAGE;Antigen;

貨號

AP50258HU

規格

100μg 500μg 1mg

價格

查詢

貨期

3-5 weeks

說明書

登陸
產品概述:
FGF basic is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation.Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21-23 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells.The 18 kDa rat sequence has 98% aa identity with mouse, and 96 - 97% identity with human, bovine and sheep FGF basic.

使用方法:
Reconstitute in PBS or others.
穩定性:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存儲:
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.

存儲緩沖液:
Tris-based buffer, 50% glycerol
注意事項:
This product is for research use only, not for diagnostic or therapeutic use.
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