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Bovine Calprotectin (CALPRO) ELISA Kit


種屬

Bovine (Bos taurus; Cattle)

UniProt號

N/A

縮寫

CALPRO

別名

CAL; MRP-8/MRP-14; S100A8/A9

線性范圍

Request Information

靈敏度

Request Information

樣本類型

Serum, Plasma, Other biological fluids

檢測方法

Sandwich

分析方法

Quantitive

反應時間

1-4.5h

樣本體積

1-200 μL

檢測波長

450 nm

貨號

AE64464BO

規格

96T 48T

價格

查詢

貨期

3-5 working days

說明書

登陸
原理:
This assay employs a two-site sandwich ELISA to quantitate CALPRO in samples. An antibody specific for CALPRO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CALPRO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CALPRO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CALPRO bound in the initial step. The color development is stopped and the intensity of the color is measured.
產品概述:
Calprotectin is a protein belonging to the S100 family and occurring in large amounts in neutrophil granulocytes, where it accounts for 5% of total proteins and 60% of cytoplasm proteins. Smaller amounts of calprotectin has also been detected in monocytes and activated macrophages. The structure of calprotectin consists of a light polypeptide chain and two heavy polypeptide chains, with a molecular weight of 36,5 kDa. Bacteriostatic and mycostatic properties of calprotectin are comparable to those of antibiotics. For this reason the abundance of calprotectin in neutrophil granulocytes and its antimicrobial activity suggest a substantial role in the defence of the organism.Calprotectin has been found in several human biological materials: serum, saliva, cerebrospinal fluid and urine.

組份:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1?x 20 mL

Biotin-Conjugate (concentrate?100 x)

1 x?120?μL

Biotin-Conjugate Diluent

1 x 20?mL

Streptavidin-HRP ?(concentrate?100 x)

1 x 120?μL

Streptavidin-HRP Diluent ??

1 x 20?mL

Wash Buffer (concentrate?25?x)

1 x 20?mL

Substrate Solution

1 x 12?mL

Stop Solution

1 x 10?mL

Adhesive Films

4

特異性:
This assay has high sensitivity and excellent specificity for detection of Bovine CALPRO. No significant cross-reactivity or interference between Bovine CALPRO and analogues was observed.

回收率:
Matrices listed below were spiked with certain level of recombinant Bovine CALPRO and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine CALPRO in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin?plasma

10

91-99

95

精密度:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%

線性:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine CALPRO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin?plasma

92-103%

93-102%

92-99%

91-101%

穩定性:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

樣本收集及儲存:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

注意事項:
Store at 2-8°C. Please refer to Instruction Manual.
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