首頁 // 產品中心 // 食品安全與藥物殘留酶聯免疫

Apramycin ELISA Kit


種屬

N/A

UniProt號

N/A

縮寫

Apramycin

別名

N/A

線性范圍

0.1-8.1 ppb

靈敏度

0.1 ppb

樣本類型

Tissue, liver, honey, etc.

檢測方法

Competitive ELISA

分析方法

Quantitive

反應時間

1-3h

樣本體積

10-200 μL

檢測波長

450 nm

貨號

AE81062SF

規格

48T 96T

價格

查詢

貨期

3-5 working days

說明書

登陸
原理:
This assay is based on the competitive enzyme immunoassay for the detection of Apramycin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Apramycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Apramycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Apramycin in it. This value is compared to the standard curve and the Apramycin concentration is subsequently obtained.
產品概述:

組份:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1?x 20 mL

Biotin-Conjugate (concentrate?100 x)

1 x?120?μL

Biotin-Conjugate Diluent

1 x 20?mL

Streptavidin-HRP ?(concentrate?100 x)

1 x 120?μL

Streptavidin-HRP Diluent ??

1 x 20?mL

Wash Buffer (concentrate?25?x)

1 x 20?mL

Substrate Solution

1 x 12?mL

Stop Solution

1 x 10?mL

Adhesive Films

4

特異性:

回收率:

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin?plasma

10

91-99

95

精密度:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
線性:

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin?plasma

92-103%

93-102%

92-99%

91-101%

穩定性:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
樣本收集及儲存:
Store at 2-8°C. Please refer to Instruction Manual.
注意事項:
Store at 2-8°C. Please refer to Instruction Manual.
青海今日快三走势图